Fu-Shin Yu
Fu-Shin Yu
Biography
Dr. Yu received his BS degree from Wuhan University, Hubei, China. He came to United States through the CUSBEA (China–United States Biochemistry Examination and Application) program. He pursued postdoctoral training at Massachusetts General Hospital, Harvard University and UT Southwestern Medical Center at Dallas before joining the faculty of the Schepens Eye Research Institute, Harvard Medical School in 1993. In 2001, he was recruited to the Medical College of Georgia where he was a tenured associated professor. In 2004, he came back to WSU as a full professor with tenure and Director of Research at the Kresge Eye Institute. Dr. Yu has served as a reviewer for numerous science journals. In addition, he has been a grant reviewer for the National Institute of Health (regular member, AED and DPVS), domestic private foundations, and several international governmental, notably National Science Foundation of China, and private funding agencies.
Education
- BS. Biology, Wuhan University
- Ph.D. (1988): Chemistry, Wayne State University
Postdoctoral Fellowship
- 1988-1989: Harvard Medical School, Massachusetts General Hospital, Boston, MA, USA
- 1989-1991: University of Texas Southwestern Medical Center, Dallas
Areas of Research
Corneal wound healing; ocular inflammation and infection, corneal innate immunity, diabetic peripheral neuropathy, ocular toxicity test.
Research
The Yu’s laboratory investigates the molecular mechanisms underlying corneal epithelial wound healing and diabetic neurotrophic keratopathy, using corneal organ culture and animal models. Specifically, the lab studies the interactions of sensory nerve, intraepithelial dendritic cells, and epithelial cells in the cornea during wound healing and their defects in diabetic corneas which lead to delayed epithelial wound closure and impaired sensory nerve regeneration (diabetic peripheral neuropathy). The second project of Dr. Yu’s lab is to study ocular innate immunity and protection against microbial infection. Dr. Yu and colleagues have shown that activation of Toll-like receptors, particularly TLR5 prior to infection resulted in profound mucosal protection against a broad spectrum of keratitis causing pathogens. The focus in Dr. Yu’s lab is to define the molecular mechanisms underlying these protective pathways.
Dr. Yu's research has been supported multiple R01 grants by the National Eye Institute.
Major equipment:
GENios and BioTek Synergy Microplate Reader for fluorescence, absorbance and luminescence; Electroporator; Applied Biosystems StepOne Plus Realtime PCR System; Zeiss dissecting microscope equipped with a digital camera; Zeiss Axiovert 200 Inverted microscope with SPOT advanced camera; Amersham BioSciences AKTA Prime attached to a Dell computer for protein purification; NIEDK Confoscan 4; Kadak Image Station 4000R Pro for Western Bloting/ECL images; Zeiss 30 Slid-Lamp Microscopy equipped with digital camera.
Resources: Cell lines:
HEK293A,; ARPE19; CaCO2; HEK/TLR2, 9, 4 expressing cells; HUCL; THK1; THP1; NIH3t3; MAKinase mutants (yin); APNP; PT67, ADAM; BREC; PC3; Lymphatic microvascular EC; Schnyder cells; THCE; Human corneal stroma cells; Pr HCECs DM and NL; PBABE; 3T3-HTERT; Vero; HEVEC; Salivary Gland cells (acinar, duct, myoepithelial cells); RPE immortalized; CHO; PC12; T47; Human brain vascular endothelial cells; Immortalized human BVEC, corneal keratocytes.
Transgenic and knockout mice (B6 background): Isg15-/-; EPHX2-/-; CD11c-DTR transgenic; MyD88-/-, IFNR1-/-
Selected publications
Gao N, Wu J, Li J, Dong C, Wu XY, Xiao X, Yu FS. CXCL10 Suppression of hem- and lymph-angiogenesis in inflamed corneas through MMP13. 2017. Angiogenesis in press
Dong C, Gao N, Ross BX, Yu FS ISG15 in Host Defense against Candida albicans Infection in a Mouse Model of Fungal Keratitis. 2017 IOVS, in press
Ross BX, Gao N, Cui X, Standiford TJ, Xu JJ, Yu FS. Interleukin-24 Promotes Pseudomonas Aeruginosa Keratitis in C57BL/6 Mouse Corneas. J Immunology, in press.
Singh PK, Guest GM, Kanwar M, Boss, J, Gao N, Juzych MS, Abrams GW, Yu FS, Kumar A. Zika virus infects cells lining the blood-retinal barrier and causes chorioretinal atrophy in mouse eyes. 2017 JCI Insight, in press
Gao, N., Lee, P. & Yu, F. S. Intraepithelial dendritic cells and sensory nerves are structurally associated and functional interdependent in the cornea. Sci Rep 6, 36414 (2016). PMCID:PMC5090364
Dandekar, A. et al. Toll-like Receptor (TLR) Signaling Interacts with CREBH to Modulate High-density Lipoprotein (HDL) in Response to Bacterial Endotoxin. J Biol Chem 291, 23149-23158 (2016). PMCID: PMC5087733
Yan C, Gao N, Sun H, Yin J, Lee P, Zhou L, Fan X, and Yu FS. Targeting Imbalance between Interleukin-1β and IL-1 Receptor Antagonist Ameliorates Delayed Epithelium Wound Healing in Diabetic Mouse Corneas. Am J Pathol. 2016;186(6):1466-80. PMCID:PMC4901143
Sun H, Mi XF, Gao N, Yan CX, and Yu FS. Hyperglycemia-Suppressed Expression of Serpine1 Contributes to Delayed Epithelial Wound Healing in Diabetic Mouse Corneas. Invest. Ophthalmol. Vis. Sci.. 2015; 56(5):3383-3392. PMCID:PMC4567624
Gao N, Kumar A, and Yu FS. Matrix Metalloproteinase-13 as a Target for Suppressing Corneal Ulceration Caused by Pseudomonas aeruginosa Infection. The Journal of infectious diseases. 2015;212(1):116-27. PMCID:PMC4481612
Liu X, Gao N, Dong C, Zhou L, Mi QS, Standiford TJ, et al. Flagellin-induced expression of CXCL10 mediates direct fungal killing and recruitment of NK cells to the cornea in response to Candida albicans infection. Eur J Immunol. 2014;44(9):2667-79. Epub 2014/06/27. doi: 10.1002/eji.201444490. PubMed PMID: 24965580; PubMed Central PMCID: PMC4165733.
Bettahi I, Sun H, Gao N, Wang F, Mi X, Chen W, et al. Genome-wide transcriptional analysis of differentially expressed genes in diabetic, healing corneal epithelial cells: hyperglycemia-suppressed TGFbeta3 expression contributes to the delay of epithelial wound healing in diabetic corneas. Diabetes. 2014;63(2):715-27. Epub 2013/12/07. doi: 10.2337/db13-1260. PubMed PMID: 24306208; PubMed Central PMCID: PMC3900551.
Yoon GS, Dong C, Gao N, Kumar A, Standiford TJ, Yu FS. Interferon regulatory factor-1 in flagellin-induced reprogramming: potential protective role of CXCL10 in cornea innate defense against Pseudomonas aeruginosa infection. Invest Ophthalmol Vis Sci. 2013;54(12):7510-21. Epub 2013/10/17. doi: 10.1167/iovs.13-12453. PubMed PMID: 24130180; PubMed Central PMCID: PMC3832217.
Gao N, Sang Yoon G, Liu X, Mi X, Chen W, Standiford TJ, et al. Genome-wide transcriptional analysis of differentially expressed genes in flagellin-pretreated mouse corneal epithelial cells in response to Pseudomonas aeruginosa: involvement of S100A8/A9. Mucosal Immunol. 2013;6(5):993-1005. Epub 2013/01/24. doi: 10.1038/mi.2012.137. PubMed PMID: 23340821; PubMed Central PMCID: PMC3722258.
Wang F, Gao N, Yin J, Yu FS. Reduced innervation and delayed re-innervation after epithelial wounding in type 2 diabetic Goto-Kakizaki rats. Am J Pathol. 2012;181(6):2058-66. Epub 2012/10/16. doi: 10.1016/j.ajpath.2012.08.029. PubMed PMID: 23063510; PubMed Central PMCID: PMC3509759.
Gao N, Yin J, Yoon GS, Mi QS, Yu FS. Dendritic cell-epithelium interplay is a determinant factor for corneal epithelial wound repair. Am J Pathol. 2011;179(5):2243-53. Epub 2011/09/20. doi: 10.1016/j.ajpath.2011.07.050. PubMed PMID: 21924232; PubMed Central PMCID: PMC3204011.